丙酮酸钙对小鼠力竭运动能力的研究

Effects of calcium pyruvate supplementation on exercise ability in mice

关键词:  丙酮酸钙  运动能力  肌/肝糖原  超氧化物歧化酶  丙二醛

Key words:  calcium pyruvate  exercise ability  liver glycogen  muscle glycogen  SOD  MDA

摘要:

［目的］探讨补充3 周丙酮酸钙对于小鼠力竭运动能力的影响。［方法］ 雄性小鼠40只，随机分为4组，分别为安静对照组、运动对照组、丙酮酸钙组、运动丙酮酸钙组。给药组每天灌胃给予600 mg/（kg·d）的丙酮酸钙共3 周。3 周末进行力竭运动实验，测各组运动至力竭时间，并取右侧腓肠肌组织和肝脏组织分别测肌糖原、肝糖原。［结果］ 与运动对照组(106.85±24.66)min比较，运动给药组（166.95±31.18）min鼠运动至力竭时间明显延长，运动能力提高，差异有显著性意义（P<0.001）。运动前丙酮酸钙组肌糖原、肝糖原含量、超氧化物歧化酶（SOD）含量明显高于安静对照组，差异有显著性意义（P<0.05）。运动力竭后各组肌糖原、肝糖原含量没有明显差异。与运动对照组比较，运动给药组SOD明显升高，差异有显著性意义（P<0.05）。运动对照组肝脏丙二醛（MDA）的含量均高于其他三组，差异有显著性意义（P<0.01）；运动给药组MDA含量明显低于运动对照组，差异有非常显著性意义（P<0.001）。血清钙浓度各组间差异无显著性意义（P>0.05）。［结论］补充3周丙酮酸钙能明显提高小鼠力竭运动能力，其可能的原因是提高运动前肌糖原和肝糖原水平。

Abstract:

［Objective］To investigate the effects of calcium pyruvate on exercise ability in mice for 3 weeks.［Methods］Forty mice were randomly divided into four groups: control group, exercise control group, pyruvate group and exercise pyruvate group .The mice in pyruvate groups received calcium pyruvate and the mice in control groups received placebo 600 mg per weight per day for 3 weeks. Exercise ability and glycogen were measured after the experiment.［Results］At the end of the experiment, there was a statistically significant improvement of exercise ability in exercise pyruvate group and an increase of the liver and muscle glycogen and SOD in pyruvate supplementation group .There was no significant difference in the liver and muscle glycogen between exercise control group and exercise pyruvate group at the end of exhaustive exercise. There was a statistically significant increase in SOD and a decrease in MDA between exercise control group and exercise pyruvate group at the end of exhaustive exercise.［Conclusions］The results suggest that supplement of calcium pyruvate for 3 weeks can improve exercise ability for mice.