急性脑梗死与3个miniSTR基因座多态性的相关研究

Correlation between polymorphism of 3 miniSTR loci and acute cerebral infarction

关键词:  急性脑梗死  miniSTR  基因多态性  等位基因

Key words:  acute cerebral infarction  miniSTR  gene polymorphisim  allele

摘要:

［目的］探讨miniSTR基因座D10S1248、D14S1434和D22S1045与急性脑梗死的相关性。［方法］ 应用荧光标记多重PCR扩增技术同时扩增3个基因座。310遗传分析仪进行毛细管电泳，用基因收集和分析软件进行数据信息的收集并确定基因型。用直接计数法计算等位基因频率，应用SPSS统计软件对病例组和对照组的各基因座的各个等位基因间进行卡方检验，计算OR值，并对有意义的OR值进行假设检验。［结果］ 基因座D10S1248和D22S1045的各个等位基因频率病例组与对照组间差异均无显著性意义。基因座D14S1434在病例组中的等位基因14在病例组和对照组间差异有显著性意义，OR值为0.433。［结论］基因座D14S1434的等位基因14可能是急性脑梗死发病的一个保护基因。

Abstract:

［Objective］Three miniSTR loci D10S1248, D14S1434 and D22S1045 were investigated, and applied to study the correlation between acute cerebral infarction and the miniSTR.［Methods］Multiple fluorescent polymerase chain reaction (PCR) within the one three loci were contained in one tube, then ABI 310 Genetic Analyzer was used to capillary electrophoresis and analyzed the length of allele fragments. Genetic data collection and analysis software was used for data collection and genotyping. Allele frequencies were calculated by direct counting. χ2 test by adopting SPSS10.0 statistical software was used to examine the difference between the cases and controls, Fisher's exact test was used for the cell data less than 5. The odds ratio was also calculated.［Results］Each of allele frequencies showed no significant difference between cases and controls in D10S1248 and D22S1045 loci. The allele 14 in D14S1434 showed significant difference between cases and controls. The odds ratios were 0.433.［Conclusions］The allele 14 in D14S1434 may be the protective gene of acute cerebral infarction.