体外肿瘤细胞培养法对非小细胞肺癌化疗药物敏感性的研究

Study on sensitivity of NSCLC to chemotherapeutic agents by tumor cells cultured in vitro

关键词:  非小细胞肺癌  MTT法  化疗药物

Key words:  non-small cell lung cancer (NSCLC)  MTT assay  chemotherapy agent

摘要:

［目的］通过常见非小细胞肺癌对不同抗癌药物敏感性作比较， 以期寻找各自的合理的化疗用药方案，从而指导不同肺癌的化疗。［方法］通过肿瘤细胞体外药敏试验（ 即四甲基偶氮唑盐着色法，MTT），对35例非小细胞肺癌（腺癌23例、磷癌12例）新鲜瘤组织原代培养进行顺铂（DDP）、卡铂（CBP）、足叶乙甙（VP-16）、阿霉素（ADM）、长春新碱（VCR）、环磷酰胺（CTX）、紫杉醇（PTX）、长春瑞滨（NVB）、金喜素（TPT）9种化疗药物敏感检测，其结果作以比较。［结果］非小细胞肺癌对PTX、TPT、ADM、CBP、NVB的高度敏感率分别为51.43%、42.86%、40.00%、37.14%、31.4%，提示肺癌对上述药物敏感，其余DDP、VCR、VP-16三种药物高度敏感率不足30%，提示治疗效果差。而CTX高度敏感率为0.00%，提示CTX治疗非小细胞肺癌不敏感。［结论］非小细胞肺癌对化疗药物的反应虽有一定的共性，但不同个体间差异有显著性意义。不同类型非小细胞肺癌对不同化疗药物的敏感性也有差别。

Abstract:

［Objective］Through comparing the chemosensitivities of advanced non-small cell lung cancer(NSCLC) to anti-cancer agents to find an reasonable drug strategy for chemotherapy and instruct the chemotherapies of different types of lung cancer.［Methods］Through in vitro assay (MTT) of sensitivities, 35 cases of NSCLC tumors (23 cases of adenocarcinoma and 12 cases of squamous carcinoma) were evaluated by 9 chemotherapeutic agents- DDP, CBP, VP-16, ADM, VCR, CTX, PTX, NVB, TPT, then the results were compared.［Results］The high response rates of NSCLC to PTX, TPT, ADM, CBP, NVB were 51.43%, 42.86%, 40.00%, 37.14%, 31.4% separately, which indicates that lung cancer was chemosensitive to those agents. The response rates of other three agents-DDP, VCR, VP-16 were less than 30%, which indicates that the therapeutic effects are bad. Whereas the high response rate of CTX was 0.00%, which indicates that NSCLC was not sensitive to CTX.［Conclusion］Although the responses of different chemotherapeutic agents are common to a certain extent, the differences among them still exist. Different types of NSCLC also show diverse chemosensitivities to anti-cancer agents.