| 引用本文: | 孙晶琳,杨 杨,娜 琴,迟小溪,包金兰,矣方圆,沙 晶,刘 矩,孟庆阳,祁兴岩,胡 军.人乳腺癌MCF-7/ADM细胞的耐药谱及brca1基因表达的实验研究[J].大连医科大学学报,2009,31(1):58-60. |
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| 人乳腺癌MCF-7/ADM细胞的耐药谱及brca1基因表达的实验研究 |
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孙晶琳1, 杨 杨1, 娜 琴1, 迟小溪1, 包金兰1, 矣方圆1, 沙 晶1, 刘 矩1, 孟庆阳1, 祁兴岩1, 胡 军2
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1.大连医科大学 临床医学专业 2003级，辽宁 大连 116044;2.大连医科大学 组织胚胎学教研室，辽宁 大连 116044
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| 摘要: |
| ［目的］ 研究人乳腺癌MCF-7/ADM细胞的耐药谱并探讨brca1基因产物表达与该细胞耐药性的相关性。［方法］经MTT法检测MCF-7/ADM细胞的耐药谱；通过免疫荧光法检测brca1基因产物表达情况。［结果］测得MCF-7/ADM细胞对阿霉素的耐药倍数为3.7倍；对长春新碱的耐药倍数为70.3倍；对氟尿嘧啶的耐药倍数为7.8倍。brca1基因产物在MCF-7/ADM细胞中的表达强度（98.23±7.63）高于MCF-7/S细胞（8.69±1.25），差异具有显著性意义（P<0.01）。［结论］MCF-7/ADM细胞是多药耐药细胞株，brca1与肿瘤多药耐药产生可能有一定的相关性。 |
| 关键词: MCF-7/ ADM细胞株 多药耐药性 brca1基因 |
| DOI：10.11724/jdmu.2009.01.15 |
| 分类号:R737.9 |
| 基金项目: |
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| Study drug-resistant spectrum of MCF-7/ADM and expression of brca1 |
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SUN Jing-lin1, YANG Yang1, NA Qin1, CHI Xiao-xi1, BAO Jin-lan1, YI Fang-yuan1, SHA Jing1, LIU Ju1, MENG Qing-yang1, QI Xing-yan1, HU Jun2
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1.Grade 2003 of Clinical Medicine, Dalian Medical University;2.Deperment of Histology and Embryology, Dalian Medical University, Dalian 116044, China
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| Abstract: |
| ［Objective］To study the drug-resistant spectrum of MCF-7/ADM cell and the relationship of the cells to brca1 protein expression.［Methods］The drug-resistant spectrum of MCF-7/ADM cell was studied by MTT assay, and then the expression of brca1 protein was detected by immunofluoresence assay.［Results］The drug-resistant folds to ADM, VCR, and 5-FU respectively were 3.7, 70.3, and 7.8. The expression of brca1 gene was high in MCF-7/ADM cells(98.23±7.63) and low in MCF-7/S cells(98.23±7.63), P<0.01.［Conclusion］MCF-7/ADM cell is multidrug resistance cell. There may be a relationship between brca1 and MDR. |
| Key words: MCF-7/ADM cell MDR brca1 |