| 引用本文: | 孙玉波,张英雪,谭仕清,朱 奇,刘陆陆,郭婷婷,封艳辉,王冬梅,赵 杰,孙艺平.蝎毒耐热蛋白对红藻氨酸诱导癫痫大鼠海马内前脑啡肽原表达的影响[J].大连医科大学学报,2009,31(1):38-41. |
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| 蝎毒耐热蛋白对红藻氨酸诱导癫痫大鼠海马内前脑啡肽原表达的影响 |
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孙玉波1, 张英雪1, 谭仕清1, 朱 奇2, 刘陆陆2, 郭婷婷2, 封艳辉3, 王冬梅4, 赵 杰3, 孙艺平4
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1.大连医科大学 2005级七年制学生;2006级医疗专业学生;3.生理学教研室;4.机能学实验室，辽宁 大连 116044
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| 摘要: |
| ［目的］ 观察蝎毒耐热蛋白对红藻氨酸（kainic acid，KA）癫痫大鼠癫痫敏感性形成的影响，并探讨前脑啡肽原（proenkephalin，PENK）在其机制中的作用。［方法］大鼠颈部皮下注射红藻氨酸，制备癫痫敏感性动物模型后，给予蝎毒耐热蛋白（scorpion venom heat resistant protein，SVHRP）进行干预。通过观察癫痫行为学表现检测SVHRP对癫痫敏感性形成的影响，同时应用RT-PCR技术检测不同处理组大鼠海马内前脑啡肽原表达的变化。［结果］蝎毒耐热蛋白干预组的大鼠较单独给予红藻氨酸组的大鼠癫痫敏感性形成率明显降低（P＜0.05），而且前脑啡肽原的表达也明显下降（P＜0.05）。［结论］蝎毒耐热蛋白能够抑制红藻氨酸诱导大鼠癫痫敏感性的形成，前脑啡肽原可能参与其作用机制。 |
| 关键词: 蝎毒耐热蛋白 前脑啡肽原 癫痫 |
| DOI：10.11724/jdmu.2009.01.09 |
| 分类号:R742.1 |
| 基金项目: |
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| Effect of scorpion venom heat-resistant protein on expression of proenkephalin in kainc acid -induced seizure rats’ hippocampus |
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SUN Yu-bo1, 1, 1, 2, 2,2, 3, 4, 41, ZHANG Ying-xue1, TAN Shi-qing1, ZHU Qi2, LIU Lu-lu2, GUO Ting-ting2, FENG Yan-hui3, WANG Dong-mei4, ZHAO Jie3, SUN Yi-ping4
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1.2005 Grade’s Students of Seven Years System;2006 Grade’s Students of Medical Treatment Major;3.Department of Physiology;4.Medical Functional Lab, Dalian Medical University, Dalian 116044, China
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| Abstract: |
| ［Objective］To investigate the effect of scorpion venom heat-resistant protein (SVHRP) on forming of kainc acid (KA)-induced epileptic susceptibility, and to explore whether proenkephalin (PENK) participates in SVHRP's antiepileptic mechanism.［Methods］Systemic injection of KA results in forming animal model of epileptic susceptibility, at the same time, treated them with SVHRP,observed the epileptic behavioral processes according Racine seizure stage, and investigated the expression of PENK mRNA by means of RT-PCR.［Results］SVHRP could obviously delay forming a time of seizure susceptibility, and decrease the expression of PENK compared with KA leads to increase the expression of PENK.［Conclusion］The results suggest that the possible mechanism of antiepileptic effects of SVHRP is that to decrease of PENK expression in the hippocampus of animal of epileptic susceptibility. |
| Key words: scorpion venom heat-resistant protein proenkephalin epilepsy |