饮食诱导非酒精性脂肪性肝病大鼠脂联素受体2基因的表达及其意义

Expression and significant of AdipoR 2 gene in liver of rats with nonalcoholic fatty liver disease induced by highly fat-enriched

关键词:  脂联素受体2  非酒精性脂肪性肝病  胰岛素抵抗

Key words:  adiponectin receptor 2 (AdipoR 2)  nonalcoholic fatty liver disease

摘要:

［目的］探讨脂联素受体-2（AdipoR 2）在饮食诱导非酒精性脂肪性肝病大鼠模型肝脏组织中的表达差异［方法］采用反转录PCR（RT-PCR）法检测饮食诱导非酒精性脂肪性肝病与对照组大鼠肝脏中的AdipoR 2 mRNA表达差异，同时检测大鼠脂联素、胰岛素抵抗指数及血糖水平。［结果］模型组大鼠肝脏组织中AdipoR 2 mRNA为0.3964±0.0988，而对照组为1.2254±0.1462，两者间差异有显著性意义（P<0.01）。模型组血糖为（5.44 ± 0.58 ）mmol/L，与对照组（5.25 ± 0.14 ）mmol/L比较差异无显著性意义；模型组脂联素水平（3.56±0.78）mg/mL，低于对照组的（4.71±0.30）mg/mL，P<0.05，而前者胰岛素抵抗指数为2.39±0.16，较后者1.32±0.30明显增高（P<0.01）。［结论］肝脏脂联素受体2表达下调可能是引起非酒精性脂肪性肝病的原因；与糖、脂肪代谢障碍，胰岛素抵抗程度提高有关。

Abstract:

［Objective］To investigate expression of AdipoR 2 in liver of rats nonalcoholic fatty liver disease induced by highly fat-enriched diet.［Methods］Using semi-quantitative RT-PVR, to measure the AdipoR 2 mRNA value of the rats with nonalcoholic fatty liver disease induced by highly fat-enriched. At same time, measure the adiponectin, insulin resistance index (IRI) and serum glucose level, and then compare these values to control group.［Results］RT-PCR analysis revealed that the expression of AdipoR 2 in hepatic liver tissue were significantly decreased in the model group 0.3964±0.0988 compared to the control group 1.2254±0.1462, P<0.01. There was no significant changing of serum glucose level between the model group, (5.44±0.58) mmol/L, compared to the control group (5.25±0.14) mmol/L. The adiponectin level of the model group was (3.56±0.78)mg/mL, lower than the normal group (4.71±0.30)mg/mL, P<0.05. Insulin resistance index of the former 2.39±0.16 was higher than the control group 1.32±0.30,P<0.01.［Conclusions］Lower expression of AdipoR 2 may be one important reason for nonalcoholic fatty liver disease induced by highly fat-enriched diet, and it has the relationship with glucose, fat metabolic disturbance and the high level of IRI.