| 摘要: |
| ［目的］肝星状细胞(HSC)活化是肝纤维化的病理基础，本研究旨在探讨整合素受体在HSC活化及肝纤维化过程中的作用。［方法］制作大鼠肝硬化模型，同时进行体外细胞株HSC-T6实验。用免疫组化法观察α平滑肌肌动蛋白（α-SMA）、整合素α5β1及纤维连接蛋白（FN）的表达。HSC细胞株在FN包被的皿上培养，测定细胞活化、α-SMA、整合素α5β1蛋白表达。［结果］(1)模型组肝组织FN、α-SMA和α5β1蛋白表达增多。(2)FN包被明星促进HSC-T6的活化、α-SMA及α5β1的表达。［结论］FN促进整合素α5β1表达是活化HSC、促进肝纤维化的重要途径。 |
| 关键词: 肝纤维化 肝星状细胞 纤维连接蛋白 整合素 |
| DOI：10.11724/jdmu.2008.06.03 |
| 分类号:R329.2 |
| 基金项目: |
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| Study of integrin α5β1 on development of rat hepatic fibrosis |
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JIANG Chun-meng1, WANG Hui-qing1, LIU Cheng-hai2
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1.Department of Gastroenterology, the Second Affiliated Hospital of Dalian Medical University, Dalian 116027, China;2.Institute of Liver Disease, Shanghai University of TCM, Shanghai 201203, China
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| Abstract: |
| ［Objective］To investigate the variation of fibronectin(FN), integrin α5β1 and hepatic stellate cells(HSC) activation during liver fibrogenesis.［Methods］Model of rat liver fibrosis and HSC cell line (HSC-T6) were employed. The expression of α-SMA, FN and integrin α5β1 protein in liver tissue were assayed. HSC-T6 cell line was cultured on uncoated or FN-coated plastic dishes. HSC activation, the expression of α-SMA and integrin α5β1 protein in HSC were determined.［Results］A high expressions of FN, α5β1 and α-SMA were observed in model group. FN increased α-SMA, and α5β1 expression of HSC-T6.［Conclusions］Increasing expression of FN and integrin α5β1 is a important machanism for activation of HSC, during development of liver fibrosis. |
| Key words: liver fibrosis hepatic stellate cell fibronectin integrin integrin |
