Aurora-A激酶在肺癌中的表达

Expression of aurora-A kinase in different human lung cancer

关键词:  Aurora-A激酶  肺癌  癌基因  过表达

Key words:  Aurora-A kinasel  ung cancer  oncogene  over expression

摘要:

［目的］探讨Aurora-A 基因在不同组织类型肺癌中的表达及其与临床的相关性。［方法］采用RT-PCR、Western-blot与免疫组化方法检测肺癌组织标本行Aurora-A的 mRNA与蛋白表达。［结果］肺癌30例标本中RT-PCR检测Aurora-A mRNA的表达，19例（63%）肺癌表达水平高于相应癌旁组织，其中Western blot方法检测20例中，13例（65%）蛋白表达水平高于相应癌旁组织。免疫组化检测48例肺癌中32例高表达（66.67%）。肺癌Aurora-A mRNA的表达水平与肺癌患者的性别，吸烟史以及病理特征无明显相关性。肺鳞癌的Aurora-A的蛋白水平高表达程度与肿瘤转移有相关性。［结论］肺癌组织Aurora-A表达明显高于其癌旁组织，提示Aurora-A基因的过表达与肺癌的发生有相关性。

Abstract:

［Objective］To study Aurora-A gene expression status of lung cancer and its influence on genesis of lung cancer in vivo.［Methods］The levels of Aurora-A mRNA and protein of lung cancer samples were detected by RT PCR, Western Blot or/and immunohistochemistry respectively.［Results］In 30 cases of lung cancer patients, the over-expressions of Aurora-A mRNA were observed in 19 cases (63%) compared with neighboring tissues (P<0.05).Western blot was adopted to assess the status of Aurora-A protein expressed in tumor in vivo from 20 patients.The over-expressioof Aurora-A protein n was detected in 13 cases (65%) compared with their neighboring tissue(P<0.05). Correspondingly, over expression were also found in 32 cases (66.67%)of 48 cases dectected by immunohistochemistry. No significant correlation existing between over expression of Aurora-A mRNA and sex, smoking history and pathological characteristics was found. On the contrary, correlation was observed between over expression level of Aurora-A protein and metastases in 48 squamous lung cancer patients.［Conclusions］The Aurora-A is highly expressed in lung cancer tissue than in neighboring tissue, suggesting the over expressed Aurora-A be one of oncogenetic factors of lung cancer.