| 摘要: |
| ［目的］确定一种源于蜗牛的壳聚糖水解酶的性质。［方法］PAHBAH法测定酶活性，电泳法测定分子量，Lineweare-Burk双倒数做图法测定酶促反应动力学特性，等电聚焦凝胶电泳测定等电点。［结果］纯化后的酶分子中外切酶的活性已经被除去，分子量为100 kD，酶的反应动力学符合Michaelis-Menten动力学规律，在25℃反应条件下，米氏常数Km为0.154 mmoL/L，最大反应速率Vmax为0.305 μmoL/L.h，等电点为5.45。［结论］确定了一种壳聚糖水解酶的性质，壳聚糖水解酶水解的壳聚糖产物壳寡糖有抗肿瘤、抗病毒的作用。 |
| 关键词: 壳聚糖水解酶 蜗牛酶 壳寡糖 |
| DOI：10.11724/jdmu.2007.05.06 |
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| 基金项目: |
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| Characterization of chitosanase from snail |
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SU li, XIN Yi, MA Yu-fang
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Dalian Medical University,Dalian 116044,China
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| Abstract: |
| ［Objective］To investigate the properties of chitosanase which comes from snail.［Methods］Detection of chitosanase activity by PAHBAH assay. Defermnatron of molecular woeight by SOS-PAGE, The exo determiation of enryme kinetic parameters by Linewear-Burk,
determinapron of isoelectric ponit by isoelectioc focusing gel electrophoresis.［Results］Through the Lineweare-Burk figures,Kinetic Km and Vmax of chitosanase were determined 0.154 mmol/L and 0.305 μmol/L.h,pI value of chitosanase were determined 5.45.［Conclusions］The properties of chitosanase is confirmed.The product of chitosanase is oligosaccharide which could inhibits tumour and virus. |
| Key words: chitosanase helicase oligosaccharide |
