引用本文:沈 洁,朴 花,于丽华,郭连英,施广霞,钱振超.榄香烯复合瘤苗HSP70-肿瘤肽活性成分的纯化及鉴定[J].大连医科大学学报,2007,29(2):97-99.
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榄香烯复合瘤苗HSP70-肿瘤肽活性成分的纯化及鉴定
沈 洁1, 朴 花2, 于丽华3, 郭连英3, 施广霞3, 钱振超3
1.大连医科大学 形态学实验室,辽宁 大连 116027;2.大连医科大学 生理学教研室,辽宁 大连 116027;3.大连医科大学 肿瘤生物治疗研究所,辽宁 大连 116027
摘要:
[目的]探讨榄香烯复合瘤苗HSP70-肿瘤肽活性成分的纯化方法。[方法]以小鼠HCa-F肝癌和L615白血病细胞为肿瘤模型,分别制备两种榄香烯复合瘤苗,通过亲和层析法制备瘤苗的HSP70-肿瘤肽活性成分,免疫印迹法鉴定两种HSP70-肿瘤肽及其活性。[结果]两种HSP70-肿瘤肽样品均在70 kD附近呈现清晰的一条HSP70-肿瘤肽蛋白带;从HCa-F瘤苗中获得的HSP70-肿瘤肽产量为(2.34±0.28)mg/109个细胞,该产量约是等细胞数L615瘤苗产量的2倍。[结论]该方法适于HSP70-肿瘤肽活性成分的提取并能保持HSP70-肿瘤肽分子的免疫活性。
关键词:  榄香烯复合瘤苗  HSP70-肿瘤肽  亲和层析法
DOI:10.11724/jdmu.2007.02.01
分类号:R73
基金项目:
Purification and identification of the active component on HSP70-tumor peptide extracted from elemene combo-tumor cell vaccine
SHEN Jie1, PIAO Hua2, YU Li-hua3, GUO Lian-ying3, SHI Guang-xia3, QIAN Zhen-chao3
1.Lab. of Morphology,;2.Department of Physiology,;3.Institute of Cancer Biotherapy, Dalian Medical University,Dalian 116027, China
Abstract:
[Objective]To investigate a purified method of the active component on HSP70-tumor peptide extracted from Elemene Combo-Tumor cell vaccine (EC-TCV).[Methods]The two of EC-TCV were prepared with cells of HCa-F hepatoma and L615 leukemia in mice respectively, then the active components on HSP70-tumor peptide were extracted from the EC-TCV with affinity chromato- graphy, and HSP70-tumor peptide and their activities were identified by Western blotting.[Results]There was a clear bend near 70 kD in each sample of HSP70-tumor peptide; the output of HSP70-tumor peptide extracted from 1×109 HCa-F cells was 2.34±0.28 mg ,the output is higher than the yield from the equal L615 by twice. [Conclusions]The method is a suitable for the extraction of HSP70-tumor peptide and preservation of the activity of HSP70-tumor peptide.
Key words:  elemene combo-TCV  HSP70-tumor peptide  affinity chromatography