| 摘要: |
| ［目的］探讨调节激活正常T细胞表达和分泌的细胞因子（RANTES）在小儿支气管哮喘发病机制中的作用。［方法］采用双抗夹心酶联免疫吸附试验（ELISA）技术检测21例哮喘急性发作期患儿和14例缓解期的哮喘患儿血清中RANTES水平，并与肺炎及支气管炎组患儿和正常对照组相比较。［结果］ ①哮喘急性发作期患儿血清RANTES水平较哮喘缓解期患儿、肺炎及支气管炎组患儿和正常对照组均明显增高(q分别为3.52, 4.54, 5.80; P分别<0.05,<0.01,<0.01)； ②哮喘缓解期患儿血清RANTES水平开始下降，与肺炎及支气管炎组和正常对照组相比已没有显著性差异（q分别为1.01, 2.70; P均>0.05）；③肺炎及支气管炎组患儿血清RANTES含量与正常对照组比较无显著性差别（q=1.80; P>0.05）。［结论］ RANTES在支气管哮喘的急性发作过程中起到一定作用。 |
| 关键词: 哮喘 儿童 RANTES |
| DOI：10.11724/jdmu.2006.06.18 |
| 分类号:R725.6 |
| 基金项目: |
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| Detection and significance of RANTES in serum of children with asthma |
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WU Jian-wen1, YU Shu-che2
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1.Dalian Children Hospital，Dalian 116012, China;2.The 2nd Affiliated Hospital of Dalian Medical University, Dalian 116027, China
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| Abstract: |
| ［Objective］To investigate the role of Regulated upon activation normal T-cell expressed and secreted (RANTES) in the pathogenesis of children's asthma. ［Methods］ The RANTES level in serum of 21 children with asthma in acute attack stage (group 1) and 14 children with asthma in relieving stage (group 2) was
detected in this study by a sandwich enzyme-linked immunoabsorbent assay and compared with that of age-matched 13 children with acute bronchopneumonia or acute bronchitis (group 3) and 8 normal children (group 4). ［Results］① The serum RANTES level of group 1 was significantly higher than that of group 2, 3 and 4
(q=3.52,4.54,5.80; P<0.05,0.01,0.01 respectively). ② Compared to children in acute attack, children with asthma in relieving stage had a low RANTES level in serum which was not significantly different from that of group 3 and 4 (q=1.01,2.70 respectively; P>0.05 for both). ③ No significant difference was found between group 3 and group 4(q=1.08; P>0.05). ［Conclusion］ RANTES plays a role in the acute attack process of asthma. |
| Key words: asthma child RANTES |
