Aurora-A激酶在人类卵巢癌细胞株中的表达研究

Expression significances of Aurora-A kinase in human ovarian carcinoma cell lines

关键词:  Aurora-A  多倍体  卵巢癌  癌基因

Key words:  Aurora-A  polyploidy  ovarian-carcinoma oncogene

摘要:

［目的］ 了解Aurora-A基因在卵巢癌细胞中的表达情况，寻找显示卵巢癌恶性程度的肿瘤标记物。［方法］采用半定量RT-PCR、Western blot检测卵巢上皮浆液性囊腺癌细胞（HO-8910）与其高转移亚株（HO-8910PM）细胞株Aurora-A 在mRNA和蛋白表达情况；借助流式细胞仪观察两种卵巢癌细胞DNA含量，并分析Aurora-A表达的相关性。［结果］两种细胞株HO-8910、HO-8910PM半定量RT-PCR结果发现，Aurora-A/β-actin比值分别为：0.95与0.98（Ｐ<0.05）； Western blot结果显示Aurora-A/β-actin比值分别为: 1.24与1.58（Ｐ<0.01）。细胞DNA 含量的检测结果，细胞含有四倍体 (4N)的细胞比例分别为21.63％和30.15％，其多倍体(>4N)细胞的比例分别为2.20%与7.16%（Ｐ<0.01）。［结论］在两种卵巢癌细胞中，Aurora-A 基因mRNA与蛋白高表达；两种细胞株Aurora-A基因表达的高低与其细胞DNA含量有相关性，尤其与异倍体的形成有关； Aurora-A基因表达卵巢癌细胞的恶性程度有关。

Abstract:

［Objective］ To investigate the expression of Aurora-A in ovarian carcinoma cell lines and to find the tumor marker which show the malignant level of ovarian carcinoma.［Methods］ The expressions of Aurora-A in ovarian carcinoma cell lines(HO-8910,HO-8910PM) in mRNA, protein level were examined by RT-PCR and Western-blot;and the association of Aurora-A expression with DNA content in two ovarian carcinoma cell lines was analyzed by flow cytometry. ［Results］ In HO-8910 and HO-8910 PM ,the ratios of Aurora-A/β-actin were 0.95 and 0.98 respectively (P<0.05) by RT-PCR;while 1.24 and 1.58 by western blot (P<0.01). The proportion of cells with 4N DNA content in HO-8910 and HO-8910PM were 21.63％ and 30.15％ respectively, the proportion with >4N DNA content were 2.20％ and 7.16％ respectively (P <0.01). ［Conclusion］Aurora-A was overexpressed in ovarian carcinoma cell lines in mRNA and protein level.The overexpression of Aurora -A in two ovarian carcinoma cell lines has correlation to the DNA content especially with the formation of the heteroploid; The expression of Aurora-A relationed to the malignancy level of ovarian carcinoma.