复方尿素霜中尿素及维生素 A 的测定

Determination of content of compound urea with ultraviolet spectrometry

关键词:  复方尿素霜  尿素  维生素A  紫外分光光度法  含量测定

Key words:  compound urea cream  urea  Vit A  ultraviolet spectrometry  content determination

摘要:

［目的］ 对复方尿素霜中尿素、维生素A的含量进行测定。［方法］ 运用紫外分光光度法。［结果］尿素的测定波长为428 nm，在20～180 μg/mL浓度范围内与吸收度呈良好的线性关系，平均回收率为99.73%，含量为（10.05±0.22）%；维生素A的测定波长为328 nm，在4～20 u/mL浓度范围内与吸收度呈良好的线性关系，平均回收率为99.41%，含量为（2.03±0.06）%，二者无相互干扰。对自制的3个批号的尿素霜中的尿素、维生素A的含量分别测定，符合外用制剂的含量要求。

Abstract:

［Objective］ This paper describes the determination of the contents of urea and VitA contained in the conpound urea cream with ultraviolet (UV) spectrometry. ［Results］ The determined wavelength of urea is 428 nm and the concentration range of 20～180 μg/mL of urea possesses the goodlinear relationship with the absorbance. The average recovery of urea is 99.73% and the content of it is (10.05±0.22)%. The VitA determination wavelength is 328 nm. VitA possesses the good linear relationship with the absorbance when within the range of 4～20 u/mL. The average recovery of VitA is 99.41% and the content o f it is (2.03±0.06)%. There is no any interference between the two determinations described above. ［Conclusion］ The contents of there batch of self-made compound creams are determined which are in accordance with the requirement of the exterior preparation.