榄香烯对多药耐药人红白血病细胞株的影响

Effect of β-elemene on multi drug resistance in K562/ADM cells

关键词:  细胞凋亡  β-榄香烯  K562/ADM细胞系

Key words:  apoptosis  β-elemene  K562/ADM cell line

摘要:

［目的］ 探讨抗肿瘤药β-榄香烯对多药耐药细胞系的影响。［方法］ 利用MTT，荧光染料Hoechst33342染色、透射电镜的方法观察β-榄香烯对多药耐药性（mdr）人红白血病细胞株K562/ADM细胞的生长和凋亡的影响。［结果 ］β-榄香烯对K562/ADM细胞的抑制作用在5～40 μg/mL的药物浓度范围内呈上升趋势，加药组24～72 h范围内细胞凋亡率不断增加，浓度40 μg/mL 72 h时达高峰62.73%±，二者呈现药物浓度和作用时间依赖性(P<0.05或P<0.01)。［结论］ 诱导细胞凋亡是β-榄香烯抗肿瘤细胞作用机制之一。

Abstract:

［Objective］ To investigate the inhibitory mechanism of β-elemene to multi drug resistance in K562/ADM cells. ［Methods］ The growth and apoptosis of K562/ADM cells treated with β-elemene was determined by MTT, hoechst 33342 staining and electron microscope(EM). ［Results］ The inhibitory affection and apoptotic rate significantly increased during treated with 5～40 μg/mL for 24～72 h of β-elemene ,the maximal inhibitory rate reached to 62.73%± after treated 72 h (P<0.05).β-elemene have obvious affection of suppression growth and induce apoptosis to K562/ADM cells , which was depended on dose of drug and treated time to some extent.［Conclusion］ Induced apoptosis is one of its resistance tumor mechanism of β-elemene.