裙带菜多糖抗肿瘤作用的研究

Studies on anti-tumor activities of Undaria Pinnatifida polysaccharides(UPPS)

关键词:  裙带菜多糖  HepG-2人肝癌细胞株  抗肿瘤活性

Key words:  Undaria Pinnatifida polysaccharides (UPPS)  human tumor cell strain HepG-2  anti-tumor activities

摘要:

［目的］观察裙带菜多糖的体内、外抗肿瘤作用，并对其机制进行讨论。［方法］采用MTT法观察裙带菜多糖体外对人肝癌细胞 HepG-2的抑制作用；通过对615荷瘤小鼠瘤重及免疫器官脾脏的研究，观察了裙带菜多糖的体内抗肿瘤作用。［结果］与阴性对照组相比裙带菜多糖各浓度组体外对人肝癌细胞 HepG-2均有很强的抑制作用（P<0.01），其中15、10 mg/mL组的抑制率分别为57.20%、51.79%，略低于5-FU组；在荷瘤小鼠实验中，与空白对照组相比裙带菜多糖各浓度组均有很强的肿瘤抑制作用，其中400、200 mg/kg组抑瘤率分别为69.13%、42.11%,与5-FU组相近。［结论］裙带菜多糖对人肝癌细胞 HepG-2抑制效果显著，小鼠体内实验也证实了裙带菜多糖较强的抗肿瘤作用。

Abstract:

［Objective］ To investigate the anti-tumor effect ofpolysaccharides from the Undaria Pinnatifida on tumor cell strain and Hca-f mice and the mechanism of anti-tumor activities was discussed at the same time .［Methods］ MTT were applied to study the anti-tumor activities of UPPS to HepG-2 cell strain in vitro ；The anti-tumor activities were observed in vivo by testing the tumor weight and the weight of spleen of Hca-f mice.［Results］ The anti-tumor activities of UPPS to HepG-2 cell strain in vitro were significantly higher than the negative controlgroup (P<0.01)and 15、10 mg/mL of UPPS inhibiting rate were 57.20%，51.79%,which were similar to 5-FU . The anti-tumor activities of UPPS in vivo were significantly higher than the negative control group and 400,200 mg/kg of UPPS inhibitingrate to Hca-f mice were 69.13%，42.11%,which were similar to 5-FU and showed potent anti-tumor activities.［Conclusions］ UPPS show potent anti-tumor activities to HepG-2 cell strain and Hca-f mice.