异搏停对小鼠瘤株Hca-A2/p化疗的影响

Effence of verapamil in chemotherapy of rats hepatocarcinma of Hca-A2/p

关键词:  肝癌  异搏停  mdr1  死亡率  抑瘤率

Key words:  hepatocarcinoma  verapamil  mdr1  mortality rate  inhibition rate of tumor

摘要:

［目的］ 观察同时应用异搏停和化疗药对荷瘤小鼠的疗效，为治疗肝癌提供理论依据。［方法］ 做荷瘤小鼠动物模型，癌瘤内多点多次注射给药，观察抑瘤率、肿瘤坏死情况、小鼠死亡率，同时用RT-PCR方法测其多药耐药基因(multidrugs resistance gene,mdr1)的表达。［结果］ 荷瘤组(LT)、化疗组(C)、异搏停组(V)、化疗药+异搏停组(CV)的mdr1分别为（13/14、14/14、3/14、1/14）；V组、CV组与LT组的mdr1 比较p<0.05 ；LT++与C++的mdr1 比较p<0.05。 C组、CV组抑瘤率分别为（35％、63.6％）与LT组比较p<0.05 ；CV组抑瘤率(63.6％)与C组(35%)比较p<0.05。C组、CV组死亡率分别为（3/20、2/20）与LT组（8/20）比较p<0.05。CV组肿瘤坏死严重。 ［结论］化疗药和异搏停同时应用较单纯化疗抗肿瘤治疗的效果为佳，提示临床化疗时应给予逆转mdr1的药物，以提高肝癌的治疗效果。

Abstract:

［Objective］ ：To offer basic theory for liver carcinoma treatment by observing the therapeutic effects of verapamil and chemotherapeutic agent in loading tumor rats. ［Methods］ Pharmaceuticals were injected in tumor of loading tumor rats in different points for many times, and then ,to observe Inhibition rate of tumor、pathology of tumor and rats of mortality and to determine expression of multidrugs resistance gene (mdr1) by RT-PCR. ［Results］ Expression of mdr1 in loading tumer group(LT) 、chemiotherapy group(C)、Verapamil group (V)、chemotherapy Verapamil group (CV) is ( 13/14、14/14、3/14、1/14) respectively and there is statistical significance between V group(or CV group) and LT group, p<0.05；between LT++ group and C++ group, p<0.05. Inhibition rate of tumor of C group、CV group is(35%,63.6%)respectively ,which compare with LT group, p<0.05；CV group compare with V group in inhibition rate of tumor, p<0.05. Rats' mortality in C group（3/20）、CV group( 2/20)compare with LT group（8/20）,p<0.05. Tumor's necrosis is outstanding in CV group. ［Conclusion］ Applying verapamil and chemotherapeutic agent together in loading rats have more effect than only chemotherapeutic agent .It indicate that reversing drugs of mdr1 and chemotherapeutic agent be applied at the same time can better therapy effects of liver carcinoma.