雌二醇促进正常宫颈上皮永生化细胞系End1/E6E7增殖的研究

Study of estrodial inducing immortalized cervical cell line Endl/E6E7 to proliferate

关键词:  雌激素受体  人乳头状瘤病毒  宫颈上皮永生化细胞

Key words:  human papillomavirus  estrogen receptor  immortalized cervical cell

摘要:

[目的 ]研究在 17- β -E2 作用下正常宫颈上皮永生化细胞End1/E6E7的生长增殖和HPV16 /E6E7基因、ERβ表达的变化 ,探讨雌激素及HPV与ERβ在宫颈上皮病变发展过程中的作用。[方法 ]选取ERα(- ) /ERβ(+ )的正常宫颈上皮永生化细胞系End1/E6E7细胞 ,应用MTT比色实验及流式细胞术研究 17- β -E2 对其生长与增殖的调控作用 ;应用RT -PCR方法半定量研究 17- β -E2 对该细胞中HPV16 /E6E7基因及ERβ基因表达的影响。 [结果 ]随 17- β -E2 浓度增加 ,倍增时间缩短 ,MTT吸光度值增高 (P =0 .0 4 5 ) ,细胞周期G0 、G1期比例下降 ,G2 、M及S期比例上升 (P =0 .0 0 2 ) ;HPV16 /E6E7mRNA表达增强 (P =0 .0 19) ;ERβmRNA表达无明显改变 (P =1.0 0 )。 [结论 ]雌激素可能通过ERβ以外的途径促进HPV16 /E6E7基因的表达 ,进而促进永生化宫颈上皮的增殖生长。

Abstract:

[Objective]To study the alterations of biological behavior and the expressions of HPV16/E6E7 and ERβ of immortalized cervical cell line End1/E6E7 affected by 17-β-E 2, and to investigate the action to estrogen and its receptor with HPV in the progression of cervical lesions. [Methods] The cytometrial curve, MTT assay and flow cytometry assay(FCM) were used to study the growth and proliferation of the cell line regulated by 17-β-estrodial when ERα was negative and ERβ was positive, and the expression of HPV16/E6E7 and ERβ were evaluated by reverse transcription-polymerase chain reaction(RT-PCR) simultaneously. [Results] The proliferation of the End1/E6E7 cell line and the expression of HPV16/E6E7 were promoted by the effect of E 2 with the concentration varying from 0.01×10 -6 M to 0.5×10 -6 M. The expression of ERβ was not altered significantly. [Conclusions] E 2 maybe activate the growth and proliferation of immortalize cervical epithelium by promoting the expression of HPV16/E6E7 with non-ERβ mechanisms.