引用本文:刘长福,安国花,魏伟,王桂兰,侯云修.久效磷农药中毒家兔血液及组织中毒质分布和再分布的研究[J].大连医科大学学报,2004,26(3):.
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久效磷农药中毒家兔血液及组织中毒质分布和再分布的研究
刘长福1, 安国花2, 魏伟3, 王桂兰1, 侯云修4
1.滨州医学院,生物化学教研室,山东,滨州,256603;2.东营市,东营区人民医院,山东,东营,25700;3.滨州医学院,解剖学教研室,山东,滨州,256603;4.滨州医学院,附属医院,急诊科,山东,滨州,256603
摘要:
应用家兔中毒模型探讨高毒类水溶性久效磷农药在动物体内分布和再分布,探讨有机磷中毒家兔体内的贮存库及有机磷中毒反跳现象发生的机理。[方法] 酶抑制法—全自动生化分析仪5,5'-二硫代双酶动力法动态测定各标本中久效磷游离毒质含量。[结果] 久效磷农药中毒6 h游离毒质含量:肾(58.53±24.69) IU/L、脑组织(77.31±44.27) IU/L、肝组织(35.43±29.70) IU/L、脂肪组织(84.61±51.48) IU/L,各组织间比较存在显著性差异(P<0.01)。久效磷农药中毒24 h游离毒质含量:肾(9.00±1.44) IU/L、脑组织(4.20±0.56) IU/L、脂肪组织(12.35±8.80) IU/L,各组织间比较存在显著性差异(P<0.01)。久效磷中毒96 h游离毒质含量:胆汁(362.81±136.62) IU/L,其他各组织均检测不出游离毒质。[结论]久效磷游离毒质在各组织中的动态分布和重分布特点各有不同。实验证明胆汁是机体内最重要的 贮存库,久效磷游离毒质于胆汁中存留时间超过96 h;有机磷中毒反跳现象与胆汁中有机磷游离毒质残留有关。
关键词:  有机磷农药  中毒  酶动力法  中毒模型  动态监测
DOI:10.11724/jdmu.2004.03.06
分类号:R446.1
基金项目:山东省卫生厅资助项目
Research on distribution and redistribution of monocrotophos in blood and tissues of poisoning domestic rabbits
LIU Chang-fu,AN Guo-hua,WEI Wei,WANG Gui-lan,HOU Yun-xiu
Department of Biochemistry, Binzhou Medical College, Binzhou 256603, China
Abstract:
[Objective] To discuss the mechanism of storage and jump back of monocrotophos in poisoning domestic rabbits. [Methods] To test free monocrotophos pesticides by 5,5'-dithionbis in each rabbit by full automatic biochemical analyzer in dynamic state. [Results] After being poisoned for 6 hours, the degrees of monocrotophos in rabbits are: kidney (58.53±24.69) IU/L, brain (77.31±44.27) IU/L, liver (35.43±29.70) IU/L, fatty (84.61±51.48) IU/L; There was a significant discrepancy among tissues (P<0.01). After being poisoned for 24 hours, the degrees of organic phosphorus in rabbits are: kidney (9.00±1.44) IU/L, brain (4.20±0.56) IU/L, fatty (12.35±8.80) IU/L; There was a significant discrepancy among tissues (P<0.01). After being poisoned for 96 h, the degrees of monocrotophos in rabbits are: bile (362.81±136.62) IU/L,while no free monocrotophos pesticides were found in other tissues. [Conclusions] The characteristics of the dynamic arrangement and arrangement of free monocrotophos pesticides in various tissues vary. It is proved the juice was the most important free monocrotophos pesticides storage in animal body. Free monocrotophos pesticides was tested even longer than 96 hours. There was a relationship between the jump back of organic phosphorus poisoning and remains of free monocrotophos pesticides in juice.
Key words:  organic phosphorus  poisoned  Enzyme power law  poisoned model  dynamic monitor