茶多酚静脉内给药对动物缺血性脑损伤的保护作用

Neuroprotective effects of iv tea polyphenol on cerebral ischemia reperfusion injury in animal models

关键词:  茶多酚  脑缺血再灌注  行为学评分  SOD活力

Key words:  tea polyphenol  ischemia reperfusion  neurological deficit score  SOD

摘要:

研究茶多酚（tea polyphenol,TP）静脉内给药对动物实验性脑缺血再灌注损伤的保护作用。［方法］1 采用断头法制备小鼠永久性全脑缺血模型，观察TP静脉内给药对断头后张口喘息时间的影响。2 采用小鼠双侧颈总动脉反复缺血再灌注模型，观察TP 对脑组织形态及脑内超氧化物岐化酶(SOD)活力的影响。3 采用线栓法制备大鼠大脑中动脉缺血再灌注损伤模型,观察TP对神经行为、存活时间及24　h存活率的影响。［结果］ 1与模型组相比，TP 100mg·kg-1可显著延长小鼠断头后张口喘息时间（0.01

Abstract:

Objective ］ To investigate the neuroprotective effects of tea polyphenol(TP) administered intravenously on cerebral ischemia reperfusion injury in rats and mice. ［Methods］ (a) Complete cerebral ischemia model was made by decapitation of mice followed by recording the gasping time. (b) The mouse ischemia-reperfusion model was made by using repeated bilateral ligation and reperfusion of common carotid arteries. The SOD activities and histological neuronal changes were measured. (c) The rat model of focal cerebral ischemia was established by occluding middle cerebral artery with nylon thread. The thread was withdrawed allowing reperfusion for 24 hours after 2-hour ischemia. The neurological deficit and survival time within 24 hours were observed. ［Results］ (a) TP100　mg·kg-1 markedly prolonged the gasping time of mice after decapitation (0.01