慢性肝炎患者外周血单个核细胞Fas抗原及T淋巴细胞亚群的检测意义

Clinical significance of Fas antigen and T lymphocyte subsets in peripheral blood mononuclear cells of patients with chronic hepatitis

关键词:  慢性肝炎  外周血单个核细胞  Fas抗原  T淋巴细胞亚群

Key words:  chronic hepatitis  peripheral blood mononuclear cells  Fas antigen  T lymphocyte subsets

摘要:

探讨Fas抗原系统和T淋巴细胞亚群在慢性肝炎发病机制中的作用及意义。［方法］提取患者外周血单个核细胞，应用流式细胞术检测Fas抗原、CD3+、CD4+、CD8+，进行统计学方差分析。共检测25例慢性肝炎患者,其中慢性乙型肝炎(CHB)患者16例，脂肪性肝炎患者9例。［结果］ 25例慢性肝炎患者总的Fas抗原及16例慢性乙型肝炎和9例脂肪肝患者的Fas抗原阳性率分别为（47.35±6.14）%、 （48.31±6.68）%和（47.19±8.71）%，明显高于对照组（25.0±7.07）%，P均< 0.01，有显著性差异。各组慢性肝病之间Fas抗原含量无显著性差异。对T淋巴细胞亚群的分析，慢性乙型肝炎组CD3+与对照组呈显著性差异（P<0.01），其余均无差异。［结论］ 慢性肝炎患者，包括CHB 及脂肪性肝炎，外周血单个核细胞Fas抗原表达明显增高，同时外周血T淋巴细胞数量（CD3+）有明显下降。

Abstract:

To investigate the possible pathogenic role of apoptosis mediated by Fas antigen system and T lymphocyte subsets in peripheral blood mononuclear cells (PBMC) of the patients with chronic hepatitis. ［Methods］ Fas antigen, CD3+, CD4+ and CD8+ of PBMC from 25 patients with chronic hepatitis, including 16 patients with chronic hepatitis B (CHB) and 9 patients with steatohepatitis, and 10 control persons were detected by flowcytometry. The relationship among these was analyzed by statistical methods. ［Results］ The Fas positive rate of PBMC in 25 patients with chronic hepatitis was （47.35±6.14）%, and those of 16 patients with CHB and 9 patients with steatohepatitis were （48.31±6.68）% and （47.19±8.71）% respectively. These rates were higher than that of control group, being （25.0±7.07）% (P<0.01). However, the positive rate of Fas antigen between two groups of chronic hepatitis showed no difference. Through analyzing T lymphocyte subsets, the significant difference was only found in CD3+ between CHB and control group. ［Conclusion］ The expression of Fas , which indicated cell apoptosis, in PBMC of patients with CHB and steatohepatitis is high ,and the number of CD3+ is decreasing.