| 本文已被：浏览 次 下载 次 |
|
|
|
| MK-801和硫酸镁对新生大鼠缺氧缺血性脑病的保护作用 |
|
何绘敏1, 曲云霞1, 隋晓燕1, 徐立新1, 侯力2, 吕申3
|
|
1.大连医科大学,第二临床学院,儿科,辽宁,大连,116027;2.大连医科大学,病理学教研室,辽宁,大连,116027;3.大连医科大学,第二临床学院,实验中心,辽宁,大连,116027
|
|
| 摘要: |
| 研究在缺氧缺血性脑病时MK-801和硫酸镁对碱性成纤维细胞生长因子（bFGF）的影响。［方法］选用新生7日大鼠制成新生儿缺氧缺血性脑病动物模型,共分为4组。(1)正常组：分离左侧颈总动脉后在动脉下置线,不结扎亦不低氧。（2）缺氧缺血处理组：模型后立即腹腔注射生理盐水0.1 mL/只。（3） MK-801处理组：模型后立即腹腔注射 MK-801 1 mg·kg-1。(4)硫酸镁处理组：模型后立即腹腔注射硫酸镁 300 mg·kg-1。上述各组每组5只。均在模型后24 h断头处死,采用免疫组化方法检测bFGF。［结果］缺氧缺血侧（左侧）大脑皮层bFGF阳性神经细胞数显著多于(P<0.01)正常组; MK-801 组及硫酸镁组。［结论］ 大脑缺氧缺血后MK-801及硫酸镁可上调bFGF，具有保护脑组织作用。 |
| 关键词: 缺氧缺血 MK-801 硫酸镁 碱性成纤维细胞生长因子 |
| DOI：10.11724/jdmu.2003.01.03 |
| 分类号:R722 |
| 基金项目: |
|
| Neuroprotection of MK-801 and magnesium sulfate in 7-day rats with hypoxic ischemic encephalathy(HIE) |
|
HE Hui-min,QU Yun-xia,SUI Xiao-yan,XU Li-xin,HOU Li,L Shen
|
|
1..Department of Pediatrics, Second Hospital，Dalian Medical University;2.Department of Pathaology， Dalian Medical University,Dalian 116027，China
|
| Abstract: |
| To study the effect of MK-801 and magnesium sulfate on bFGF treating 7-old-day rats with HIE. ［Methods］ The model of 7-old-day rats with HIE was established.This study included four groups: normal group (no hypoxia and ischemia ); HIE group(normal saline 0.1 mL/kg); MK-801 treatment group(1 mg/kg after HIE);sulfate magnate treatment group (300mg·kg-1 after HIE) . Every group had 5 rats,all rats were killed 24 h after HIE. ［Results］The 6FGF positive neural cells of HIE group were more than those of the normal group （P<0.01）, and those of MK-801 and magnesium sulfate groups were more then that of HIE group. ［Conclusion］ MK-801 and magnesium sulfate may behave protective effect on 7-old-day rat with HIE by improving bFGF |
| Key words: HIE MK-801 magnesium sulfate basic fibroblast growth factor |