NAG及mALB在肾病早期诊断中的应用

Application of NAG and mALB in diagnose of early renal disease

关键词:  N-乙酰-β-D-氨基葡萄糖苷酶  尿微量白蛋白  尿素  肾病  早期诊断

Key words:  N-acetyl-β-D-glucosaminidase  urinary microalbumin  urea  renal disease  rarly diagnosis

摘要:

探讨早期诊断肾脏损伤的方法，联合尿NAG及尿mALB两项指标对于肾病的早期诊断具有一定的价值。方法： 采用速率法测定尿NAG、血尿素（BU），Jaffe速率法测尿肌酐，免疫速率散射比浊法测尿mALB。结果：正常对照组尿NAG为(9.37±4.21)IU/gCr,尿mALB为(12±10)μg/ml，血尿素为(4.71±1.50)mmol/L，疾病组尿NAG及mALB较正常对照组显著增高（P<0.001）；糖尿病组血尿素较正常对照组显著增高（P<0.001），高血压组血尿素较正常对照组增高（P<0.05），红斑狼疮与尿路感染组血尿素较正常对照组无显著性差异。尿NAG在糖尿病、高血压、系统性红斑狼疮及尿路感染中阳性率分别达56.25%、52.17%、40%和40%，分别较血BU的阳性检出率高出34.37%、34.78%、20%和26.67%；而尿mALB在糖尿病、高血压、系统性红斑狼疮及尿路感染中阳性率分别为50%、47.82%、50%和46.67%，分别较血BU的阳性检出率高出28.12%、30.43%、30%和33.34%。结论：尿NAG及mALB是诊断肾脏早期损伤灵敏、可靠的实验室指标。

Abstract:

To provide diagnostic basis for early renal disease，Measuring both urinary NAG and urinary microalbumin can get a higher positive rate.Methods: Urinary N-acetyl-β-D-glucosaminidade (NAG)and blood urea were determined with rate,Urinary creatinine was determined with Jaffe's rate，mALB levels was measured with rate nepherometry. Results: The levels of urinary NAG and blood urea in the controls were (9.37±4.21)IU/gCr and (4.71±1.50)mmol/L,urinary mALB was (12±10)μg/ml. The level of urinary NAG and urinary microalbumin in each group (diabetes,hypertension,lupus erythematosus and uretha infection)were obviously higher than that in the control(P<0.001); Blood urea in diabetes and hypertension groups were higher than that in the control （P<0.001，P<0.05）,In lupus erythematosus and urethra infection weren't higher.Positive rates of urinary NAG in diabetes,hypertension,lupus erythematosus and urethra infection groups were 56.25%, 52.17% ,40% and 40%, respectively.They were higher than the positive rate of blood urea by 34.37%, 34.78%,20.% and 26.67%, respectively. Positive rates of urinary microalbumin in diabetes,hypertension,lupus erythematosus and urethra infection groups were 50%,47.82%, 50% and 46.67%，respectively. They were higher than the positive rate of blood urea by 28.12%, 30.43%, 30.% and 33.34%, respectively. Conclusion: The urinary NAG and urinary mALB is sensitive and reliable for diagnose of early renal disease