胆道梗阻后心肌损伤及川芎嗪保护作用的实验研究

Experimental study on myocardial injury and the protective effects of tetramethylpyrazine after biliary obstruction

关键词:  胆道梗阻  心肌  自由基  肿瘤坏死因子  川芎嗪

Key words:  biliary obstruction  myocardium  free radical  TNFα  tetramethylpyrazine (TMP)

摘要:

观察胆道梗阻后心肌损伤及川芎嗪(TMP)的保护作用。方法:复制大鼠胆道梗阻模型，每日予TMP 30mg/kg注射，动态观测心肌组织丙二醛(MDA)和超氧化物歧化酶(SOD)含量、血清T-Bil、TBA、内毒素(ET)、肿瘤坏死因子(TNFα)含量；行光电镜检查，应用ABC免疫组化染色法，定位TNFα在心肌组织中的表达和分布。结果：胆道梗阻后，血清T-Bil、TBA、ET、TNFα水平逐渐升高，心肌组织MDA含量逐渐升高，SOD逐渐减少，各梗阻组与对照组比较，P＜0.05；各TMP治疗组与同时相梗阻组比较，血清T-Bil、TBA、ET、TNFα水平下降(P＜0.05)，心肌组织MDA含量减少(P＜0.05)，SOD含量升高(P＜0.05)。结论:自由基损伤、内毒素血症、TNFα的综合作用是胆道梗阻所致心肌损害的主要机制，TMP对胆道梗阻所致心肌损伤确有保护作用

Abstract:

To investigate myocardial injury and the protective effects of tetramethylpyrazine (TMP) after biliary obstruction. Methods: The TMP was injected to peritoneum of the rat once a day at a dose of 30 mg/kg after establishment the model of biliary obstruction. The contents of myocardial malondialdyhyde (MDA) and superoxide dismutase(SOD), the levels of serum T-Bil、TBA、endotoxin(ET) 、tumor necrosis factor(TNFα) were determined . Left ventricle myocardium was drawn for optical and electronic microcope observation. Immunohisto-chemical staining method of ABC was to locate the expression and distribution of TNFαin myocardial tissues. Result: After BDL, serum T-Bil、TBA、ET、TNFα levels and myocardium MDA were increased significantly, while SOD decreased markedly as compared with control group (P＜0.05); As compared with BDL group at the same obstructed phase, serum T-Bil、TBA、ET、TNFα levels and myocardium MDA in TMP-treated groups were decreased significantly (P＜0.05), while myocardium SOD increased significantly (P＜0.05). The myocardium MDA content was highly positively correlated with serum TNFα　levels. There were less myocardial injuries in TMP-treated groups than those in BDL groups at the same stage by electronic microscopy observation. Conclusion: ET、TNFα and myocardial free radical injury may be the important mechanisms of myocardial injury induced by biliary obstruction; TMP can protect myocardium effectively from the injury after biliary obstruction.