20(R)-人参皂甙Rg3对K562/ADM细胞凋亡诱导的研究

Tumer cells in K562/ADM cell line might be induced to apoptosis by 20(R)-Ginsenoside Rg3

关键词:  20(R)-人参皂甙Rg3  凋亡  电镜

Key words:  20(R)-Ginsenoside Rg3  apoptosis

摘要:

探讨抗癌药物20(R)-人参皂甙Rg3(20(R)-Ginsenoside Rg3)对肿瘤细胞凋亡的诱导。方法：采用人红白血病耐阿霉素（adriamycin，ADM）细胞株K562/ADM细胞作为实验模型,用ADM作阳性对照。结果：MTT法细胞毒实验显示，Rg3对K562/ADM细胞的IC 50为10.49±0.30 μg/ml；流式细胞仪法测定该细胞与浓度为1.0 μg/ml(无毒剂量)和5.0 μg/ml(低毒剂量)的Rg3共同培养48 h的细胞凋亡率分别为3.39±0.25%和9.36±0.61%；电镜下，可见凋亡的K562/ADM细胞及凋亡小体。结论：Rg3具有较强的诱导肿瘤细胞凋亡的能力，其诱导强度与其浓度呈正相关，对作用时间有依赖性

Abstract:

To investigate apoptosis of tumor cells induced by 20(R)-Ginsenoside Rg3.Methods: The human erythroleukemia variant cell K562/ADM that has resistance to adriamycin(ADM) was used an experimental model. The toxicity of Rg3 to K562/ADM cell was tested by method MTT; The apoptosis rate was detected with flow cytometry(FCM); Transmission electron microscope (TEM) was used to find out Apoptotic bodies. Results: The IC50 value of Rg3 on K562/ADM cell was 10.49±0.30 μg/ml;The apoptosis rates of K562/ADM cells with Rg3 1.0 μg/ml(innocurity) and 5.0 μg/ml(low toxicity) were 3.39±0.25% and 9.36±0.61% respectively after cultured for 48 h; Apoptotic bodies of K562/ADM cell were seen by TEM.Conclusions: Rg3 has more powerful ability to induce tumor cell to apotosis; The strength of apoptosis induction of Rg3 for K562/ADM cells is positively relative to the concentrations of Rg3 and has dependence on time.