| 摘要: |
| 研究蝎毒对小鼠淋巴瘤细胞株的生长抑制和诱导凋亡作用。方法：应用MTT法观察蝎毒对细胞的抑制作用，光镜、透射电镜观察细胞结构的改变，原位末端标记检测DNA断裂，流式细胞术分析凋亡细胞。结果：蝎毒(12.5-200μg/ml)可明显地抑制小鼠淋巴瘤细胞生长和诱导细胞凋亡,并且其作用强度在一定范围内呈现对浓度和时间的依赖性。结论：蝎毒能抑制小鼠淋巴瘤细胞生长和诱导细胞凋亡。 |
| 关键词: 蝎毒 细胞凋亡 小鼠淋巴瘤细胞株 |
| DOI：10.11724/jdmu.2001.03.02 |
| 分类号:R733 |
| 基金项目: |
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| Studies of BMK on inhibition of cell growth and induction of apoptosis in Yac-1 cell line |
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JIA Li,MENG Xiu-xiang,SUN Hong-dan,SONG Zhen-lan
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Dalian Medical University,Dalian116027,China
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| Abstract: |
| The study of the BMK on inhibition of cell growthand induction of apoptosis in Yac-1 cell line. Methods: The growth inhibition was analysed by MTT assay, cell apoptosis by light microscopy, transmission electron microscopy, flow cytometry and TUNEL technique. Results: The results showed that BMK(12.5-200 μg/ml) could inhibit cell growth and induce apoptosis obviously, and the effect of BMK was dose- and time-dependent within a limited range. Conclusions: BMK could inhibit cell growth and induce apoptosis obviously. |
| Key words: BMK apoptosis Yac-1 cell line |