| 摘要: |
| 目的:筛选产酶菌株并探讨其最佳产酶条件.结果:其中的一株无根根霉(Rhizopus arrhizus Fischer-29)显示了最高的脂肪酶酶活力.它的最适产酶条件为培养基初始pH 7.0,鱼油加入量1%,32℃.摇瓶发酵48h,最高发酵液酶活34.98u/ml,在此条件下水解鱼油,可使其中的DHA、EPA的含量由原来的10%提高到40%,是任何传统方法所无法比拟的,适合工业化生产. |
| 关键词: 微生物脂肪酶 Rhizopus arrhizus Fischer |
| DOI：10.11724/jdmu.2001.02.32 |
| 分类号:R392 |
| 基金项目: |
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| Study on the sieve and optimal culture conditions of Rhizopus arrhizus Fischer Lipase - 29 |
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SUN Hong-dan1, HU Ju2, YAO Min-chang3
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1.Department of Clinical Hemotology,Dalian Medical University,Dalian 116027,China;2..hedical collegt of Dalian university Dalian 116600,china;3.Department of Biology,Liaoning Normal University, Dalian 116022,China)
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| Abstract: |
| A strain of mold Rhizopus arrhizus Fischer Lipase -29 producing Lipase has been discussed. The optimal culture conditions for medium which was used to culture Rhixopus arrhizus Fischer Lipase-29 were pH 7.0,fish oil 1%,32℃ and shaking 48 h. The most hydrolysis activity of culture media was 34.98 u/ml |
| Key words: microorganic lipase Rhizopus arrhizus Fischer |