| 摘要: |
| 应用高效液相色谱法同时测定了栀子中京尼平甙和京尼平的含量.采用YWG-C18柱,0.05mol/L磷酸氢二钠-甲醇(72∶28),用磷酸调节pH6.80为流动相,于240nm波长处检测,京尼平甙和京尼平的平均加样回收率分别为99.10%,98.28%,RSD分别为1.05%,1.21%(n=5).本法简单、准确、重现性好. |
| 关键词: 高效液相色谱 京尼平甙 京尼平 栀子 |
| DOI：10.11724/jdmu.2001.01.25 |
| 分类号:R-331 |
| 基金项目: |
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| Simultaneous Determination of Geniposide and Genipin in Gardenia Jasminoides Eills by High Performance Liquid Chromatography |
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CAO Jing-ping1, WANG Yan-lei2, JIA Yu-jie2
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1.Central Research Lab.of Dalian Medical University, Dalian, 116027 China;2.Department of pathopgysiology, Dalian Medical University
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| Abstract: |
| A new method for the simultaneous determination of genooposide and genipin in Gardenia jasminoides Eills by reversed-phase high perfomance liquid chromatography was studied. The method used a YWG-C18 column, 0.05 mol/L di-sodium hydrogen phosphate-methanol (72∶28) as the mobile phase at a flow rate of 1.0 ml/min with detection wavelengty at 240 nm. Retention times and recovery rates for geniposide and genipin were 6.33,9.49 min,and 99.10%, 98.28%, respectively. The RSD of the results was 1.05% for geniposide, 1.21% for genipin. The method was proved to be simple and reliable. |
| Key words: reversed-phase high performance liquid chromatography gardenia jasminoides Eill geniposide genopin |