MgSO4对大鼠脑缺血再灌流损伤后Na+－K+－ATP酶活性、NO的影响

The preventive effects of MgSO4 during cerebral ischemia-reperfusion

关键词:  脑缺血  再灌流损伤  Na+－K+－ATP酶  NO  MgSO4

Key words:  cerebral ischemia-reperfusion injury  NO  MgSO

摘要:

讨MgSO4对大鼠脑缺血再灌流损伤的保护作用。方法：采用线栓法将SD大鼠制成脑缺血再灌流模型，分组测定钠泵(Na+－K+－ATP酶)活性、一氧化氮(NO)含量、脑含水量以及镁剂对其影响。结果：对照组与假手术组比较：Na+－K+－ATP酶活性降低，NO含量与脑含水量增加，且均有显著性；治疗组(500 mg/kg MgSO4)与对照组比较：Na+－K+－ATP酶活性增加、NO含量及脑含水量降低，且均有显著性。结论：MgSO4能提高大鼠脑缺血再灌流损伤后Na+－K+－ATP酶活性，降低NO含量及脑含水量，对脑损伤有保护作用

Abstract:

Objective:To explore therapeutic effects of magnesium sulfate(MgSO4) during cerebral ischemia-reperfusion.Methods:Using an intraluminal thread method, a cerebral ischemia-model (Middle Cerebral Artery Occlusion,MCAO)of rat was developed. To observe the cerebral Na+－K+－ATP ase activity changes, nitricoxide(NO) levels and contents of water in brain at 3 hours reperfusion following 2 hours cerebral ischemia,40 Sprague-Dawley(SD)rats were randomly divided into sham-operated group(10 rats),control group(15 rats)and treatment group(15 rats), and MgSO4(500 mg/kg) was administered in treatment group at the beginning of reperfusion. Results:The cerebral Na+－K+－ATPase activity decreased(P＜0.01), and NO levels and contents of water in brain increased significantly (P＜0.01) in the control group, compared with sham-operated group. The cerebral Na+－K+－ATPase activity increased(P＜0.01), NO levels and contents of water in brain decreased(P＜0.01) significantly in the treatment group compared with control group. Conclusion: MgSO4 administered may prevent brain injury from cerebral ischemia-reperfusion.