| 摘要: |
| 用EcoR I/Xho I两种限制性内切酶在同一反应体系中对TGFβ1cDNA重组质粒联合酶切获得1.3Kb的酶切片段作为裸探针，比以往文献中报道的长度缩短约700 bp，经地高辛标记获得较高的标记效率。本方法简化了操作步骤，并大大降低了cDNA探针的制备成本，不失为制备TGFβ1cDNA探针的好方法，为TGFβ1mRNA的检测提供了较好的工具。 |
| 关键词: |
| DOI：10.11724/jdmu.2000.02.28 |
| 分类号: |
| 基金项目: |
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| The preparation of digoxin labeled cDNA probe of human TGFβ1 |
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QI Zan-mei,ZHAI Ling,L Chang-long
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(Department of Immunology,China Medical University,Shenyang 110001,China)
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| Abstract: |
| In this study we used a 1.3 Kb longDNA fragment as nude probe which was obtained from the recombinant plasmid of TGFTGFβ1cDNA with the combine incision of two endonuclease EcoR I/Xho I in the same reaction system.This probe is shorter by 700 bp than those early reported and is labeled by digoxin with high efficiency.This method not only simplified the operation teps,but also reduced the cost of the preparation of cDNA probe.therefore,it can be regarded as a good measure for the preparation of TGFβ1cDNA probe,and provides a good mean for the detection of TGFβ1mRNA. |
| Key words: TGF β cDNA probe digoxin labelled |