栓线长度、直径及大鼠体重与栓线法大鼠局灶性脑缺血模型关系的研究

The relation of rat MCAO model quality to thread diameter, length and body weight of the rats

关键词:  栓线插入长度  栓线直径  体重  局灶性脑缺血  动物模型  大鼠

Key words:  Animal model  cerebral ischemia  focal nylon monofilament length  Nylon monofilament diameter  body weight  rats.

摘要:

为提高栓线法大鼠局灶性脑缺血(MCAO)模型的成功率和稳定性，探讨大鼠体重、栓线插入长度和直径与MCAO模型成功的关系。方法：利用2×2×2析因设计，通过检测神经功能缺陷评分和脑梗塞体积，分析大鼠体重、栓线插入长度和直径与MCAO模型成功的关系。结果：大鼠体重、栓线直径两水平之间有差异(P＜0.05)，栓线插入长度两水平之间无差异(P＜0.05)；大鼠体重、栓线直径之间有正交互作用(P＜0.05)，其余两两之间以及三者之间无交互作用(P＞0.05)。结论：最佳造模方案是大鼠体重250～300 g、线长17mm或18mm、线直径0.28 mm，该方案制作的MCAO模型成功率高，重复性好。

Abstract:

Objective:To clarify the relation of middle cerebral artery occlusion ［MCAO(intraluminal thread method)］model to the filament diameter and length and rat weight.Methods:The relation of the filament diameter and length,rat weight was analyzed with 2×2×2 factorial design,via assessing neurlolgical deficit scores.Results:The neurological deficit scores betwwen the two levels of the rat weight or the fliament diameter were significantly different (P＜0.05). No significant difference was found between the two levels of the filament length(P＞0.05). A positive interaction was shown between the rat weight and the filament diameter(P＜0.05),but no interaction among other factors.Conclu-sion:The reliability and reproducibilty of this MCAO model were greatly improved,when following the optimal method(rat weight 250g-300g,filament diameter 028 mm and filament length 17 mm or 18 mm.