胃粘膜PCNA、p53表达与Hp感染及癌变的关系研究

PCNA and p53 protein expression in gastric mucosal lesions and their relationship to Hp infection and carcinogenesis

关键词:  增殖细胞核抗原  突变型p53蛋白  幽门螺杆菌  癌变

Key words:  PCNA  p53  Hp  carcinogenesis

摘要:

探讨胃上皮细胞增殖与幽门螺杆菌(Hp)感染的关系及Hp感染致癌的可能机制。方法：应用快速尿素酶试验和WS银染色法将各种病变分为Hp感染阳性组和阴性组，应用免疫组化SP法对各组病变的增殖细胞核抗原(PCNA)、p53表达进行检测。结果：总体上看，Hp阳性组PCNA表达的阳性率明显高于Hp阴性组(P＜0.005)；且Hp阳性组总体p53表达的阳性率明显高于Hp阴性组总体(P＜0.05)；p53表达的阳性率随病变程度的加重而逐渐增加；但PCNA表达与胃粘膜炎症程度无相关性(P＞0.05)。结论：Hp感染可促进胃上皮细胞过度增殖，这种过度增殖可能是Hp感染致胃癌的机制之一

Abstract:

To obsetve the relationship between cell proliferation and Hp infection. ②To further elucidate the possible pathogenesis of Hp infection in gastric carcinoma. Methods: The expressions of PCNA andp53 in gastric epithelial cell were examined with SP technique immunohistochemically and divided into four grade(－,+,++，+++)according to percentage and staining intensity of positive cell. Results: ①Of all patients,the expressions of PCNA in the Hp Positive group were significantly higher than that in the Hp negative group(P＜0.005); The expression of p53 in the Hp positive group were significantly higher than that in the Hp negative group(P＜0.05). ②The expression of p53 was increased from CSG,CAG,IM to Dys,and teached a peak in GCa.③There was no significant difference in expressions of PCNA among light,middle and severe degrees of gastric mucosal inflammation(P＞0.05).Conclusions:Hp infection,as a risk factor for gastric carcinoma,is associated with proliferation of gastric epithelial cells, which is one of the pathogenesis of gastric carcinoma