| 摘要: |
| p53基因突变是肿瘤发生中的多发事件，导入野生型p53基因能抑制肿瘤细胞的生长。通过腺病毒载体介导，将野生型p53基因转导到人肺腺癌细胞系(GLC-82)中，证实对肺腺癌细胞生长具有明显的抑制作用，抑制率达61%。对GLC-82细胞系裸鼠皮下移植瘤进行治疗实验结果表明，p53能抑制肿瘤细胞体内的生长。流式细胞计数及DNA片段化分析显示，p53基因的转导能诱导肺腺癌细胞发生凋亡。研究结果提示，腺病毒能介导p53基因对肿瘤进行治疗，并可作为肿瘤外科手术、化疗、放疗外的又一治疗方法 |
| 关键词: p53基因 腺病毒载体 人肺腺癌细胞 肿瘤基因治疗 |
| DOI：10.11724/jdmu.2000.02.02 |
| 分类号:R73.3 |
| 基金项目: |
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| The growth suppression of human lung adenocarcinoma cell by the introduction of the Wild-type p53 gene via a recombinant abenovirus |
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XING Rong1, LIN Chen2, CHENG Jin-ke2
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1.Department of Pathophysiology,Dalian Medical University,Da lian 116027,China;2.Department of Cell Biology and National Laboratory of Molecular Oncology,Caccer Institute,Chinese Academy of Medical Science s,Peking Union Medical College, Beijing 100021,China)
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| Abstract: |
| In our research,the wild-type p53 gene was transferred into human lung adenocarcinoma cell line(GLC-82)and reduced the growth of GLC-82 cells in vivo and in vitr(the inhibiting rate:6 1% and 42%).The other results demonstrated that adenovirus-mediated tran sfer and espression of the wild-type p53 in GLC-82 lacking ghe p53 g ene resulted in GLC-82 cells apoptosis which was indicated by FCM and DNA fragment analysis.These results can offer yome scientific evidence s for use of the wild-type. p53 gene to tumor gene therapy. |
| Key words: p53 adenovirus vector GLC-82 cell line tumor gene therapy |